In the present article, an attempt has been made to provide a fully operational and useful guide regarding the implementation of two-stage cluster sampling plan in field research, which is one of the most widely used sampling plans according to the current conditions and facilities in the country. . In addition, for further use by researchers in this field, this article provides codes for implementing two-stage cluster sampling with unequal size based on probability proportional to size along with an example on two-stage cluster sampling with probability proportional to size (PPS) with hypothetical data using R software is included.